Mount Sinai researchers have revealed new insights into how the body regulates craniofacial development in newborns, which sometimes can lead to birth defects such as cleft lip or palate.
The study focused on fibroblast growth factors (FGFs), a family of growth factors that mediate cellular responses, in mice. The researchers studied signaling pathways that influence cell behavior and also reported that biological processes beyond just signaling, particularly cell adhesion, might play a pivotal role in how FGFs regulate a host of pathologies.
FGFs bind to and activate four different molecules called receptor tyrosine kinases (RTKs) on the cell surface. In turn, these RTKs trigger an established signaling pathway that influences cellular behaviors such as proliferation, death, and migration.
Improper activation of these receptors and aberrant signaling within these so-called signal transduction pathways have been linked to skeletal birth defects of the face and jaw and to premature bone formation in the sutures, where the fibrous joints between the bones of a baby’s skull fuse before the brain is fully formed, giving the head a misshapen appearance.
Deregulation of FGF activity also has been linked to multiple forms of cancer.
“Through our laboratory work with mice, we’ve elucidated for the first time the unique role of signaling pathways that are engaged downstream by the FGF receptors in embryonic development,” said Philippe Soriano, PhD, professor of cell, development, and regenerative biology at the Icahn School of Medicine at Mount Sinai and senior author.
“This is revealing because these signaling mechanisms and the phenotypic consequences of their disruption are giving us a better understanding of how FGFs affect mid-face closure and development of the jaw. In the mouse, FGF receptors also affect implantation of the embryo into the uterus,” said Soriano.
Over the years, the researchers said, Soriano’s laboratory has played a key role in unraveling the mechanism of RTK using genetic approaches in mice as a model system.
The study also breaks new ground by uncovering how RTKs may function beyond their well-known roles in cell signaling, the researchers added. By engineering mutant mice that express receptors unable to engage the classic signaling pathways, the researchers were able to identify how FGFs regulate cell adhesion, the process by which cells attach to each other or to the extracellular matrix, which provides structural and biochemical support for surrounding cells.
“We have always thought that all FGF activities are dependent on the typical established signaling pathways,” said Soriano. “But we were able to identify new signaling outputs that seem to function in ways independent of FGF signal transduction pathways. One of those outputs is cell adhesion.”
The researchers are continuing to investigate how FGF receptors work not just on the surface of the cell, which is established science, but within the cell to modulate how cells stick to each other or to the extracellular matrix. Giving impetus to their work is the fact that knowing precisely how FGFs regulate cell adhesion could open a valuable window for scientists onto a process that is believed to underlie the development of many types of cancer, they said.
“Perhaps most importantly, we’ve created surprising new investigative channels through our findings with regard to cell adhesion and signaling pathways,” said Soriano. “We now want to know if there are additional biological processes at play that could bring us closer to the development one day of inhibitors of these various pathways that might prevent diseases in which FGFs and their receptors are believed to be complicit.”
The study, “FGF Signaling Regulates Development by Processes Beyond Canonical Pathways,” was published by Genes & Development.
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